Cabazitaxel overcomes cisplatin resistance in germ cell tumour cells.

ABSTRACT:

Background

Cisplatin-based chemotherapy is highly effective in metastasized germ cell tumours (GCT). However, 10-30 % of patients develop resistance to cisplatin, requiring salvage therapy. We investigated the in vitro activity of paclitaxel and the novel taxane cabazitaxel in cisplatin-sensitive and -resistant GCT cell lines.

Methods

In vitro activity of paclitaxel and cabazitaxel was determined by proliferation assays, and mode of action of cabazitaxel was assessed by western blotting and two screening approaches, i.e. whole proteome analysis and a human apoptosis array.

Results

Activity of paclitaxel and cabazitaxel was not affected by cisplatin resistance, suggesting that there is no cross-resistance between these agents in vitro. Cabazitaxel treatment showed a st

SUBMITTER: Gerwing M 

PROVIDER: S-EPMC11818977 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature