MiR-181a promotes G1/S transition and cell proliferation in pediatric acute myeloid leukemia by targeting ATM.

ABSTRACT:

Purpose

Abnormal expression of miRNAs is intimately related to a variety of human cancers. The purpose of this study is to confirm the expression of miR-181a and elucidate its physiological function and mechanism in pediatric acute myeloid leukemia (AML).

Methods

Pediatric AML patients and healthy controls were enrolled, and the expression of miR-181a and ataxia telangiectasia mutated (ATM) in tissues were examined using quantitative PCR. Moreover, cell proliferation and cell cycle were evaluated in several cell lines (HL60, NB4 and K562) by using flow cytometry after transfected with miR-181a mimics and inhibitors, or ATM siRNA and control siRNA. Finally, ATM as the potential target protein of miR-181a was examined.

Results

We found that miR-181a was significantly in

SUBMITTER: Liu X 

PROVIDER: S-EPMC11819228 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature