Fabrication and Characterization of Electrospun DegraPol^® Tubes Releasing TIMP-1 Protein to Modulate Tendon Healing.

ABSTRACT:

Background

Tendon rupture repair can result from fibrotic scar formation through imbalanced ECM deposition during remodeling. The tissue inhibitors of matrix metalloprotease (TIMPs) not only decrease ECM degradation, regulated by matrix metalloproteases (MMPs), but also restrict TGF-β1 activation and thus diminish fibrosis.

Methods

Rabbit tenocytes (rbTenocytes) and rabbit adipose-derived stem cells (rbASCs) were cultivated under different TIMP-1 concentrations. Proliferation and gene expression were assessed. TIMP-1 was incorporated into emulsion electrospun DegraPol^® (DP) tubes that were characterized by SEM for fiber thickness, pore size, and wall thickness. Static and dynamic water contact angles, FTIR spectra, and TIMP-1 release kinetics were determined.

R

SUBMITTER: Rieber J 

PROVIDER: S-EPMC11820012 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature