A molecular proximity sensor based on an engineered, dual-component guide RNA.

ABSTRACT: One of the goals of synthetic biology is to enable the design of arbitrary molecular circuits with programmable inputs and outputs. Such circuits bridge the properties of electronic and natural circuits, processing information in a predictable manner within living cells. Genome editing is a potentially powerful component of synthetic molecular circuits, whether for modulating the expression of a target gene or for stably recording information to genomic DNA. However, programming molecular events such as protein-protein interactions or induced proximity as triggers for genome editing remains challenging. Here, we demonstrate a strategy termed 'P3 editing', which links protein-protein proximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA. By eng

SUBMITTER: Choi J 

PROVIDER: S-EPMC11820115 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature