Glucokinase activity controls peripherally located subpopulations of β-cells that lead islet Ca²⁺ oscillations.

ABSTRACT: Oscillations in insulin secretion, driven by islet Ca²⁺ waves, are crucial for glycemic control. Prior studies, performed with single-plane imaging, suggest that subpopulations of electrically coupled β-cells have privileged roles in leading and coordinating the propagation of Ca²⁺ waves. Here, we used three-dimensional (3D) light-sheet imaging to analyze the location and Ca²⁺ activity of single β-cells within the entire islet at >2 Hz. In contrast with single-plane studies, 3D network analysis indicates that the most highly synchronized β-cells are located at the islet center, and remain regionally but not cellularly stable between oscillations. This subpopulation, which includes 'hub cells', is insensitive to changes in fuel metabolism induced by glucokin

SUBMITTER: Jin E 

PROVIDER: S-EPMC11820133 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature