Rapid and Efficient Synthesis of Succinated Thiol Compounds via Maleic Anhydride Derivatization.

ABSTRACT: Succination is a non-enzymatic post-translational modification of cysteine (Cys) residues, resulting in the formation of S-(2-succino)cysteine (2SC). While hundreds of 2SC-modified proteins have been identified and are associated with the dysfunction of proteins, the underlying molecular mechanisms remain poorly understood. Conventional methods for synthesizing succinated compounds, such as 2SC, often require prolonged reaction times and/or HCl hydrolysis. In this study, we present a rapid and efficient synthesis method for succinated compounds using maleic anhydride, enabling more effective in vivo studies of succination mechanisms. This method was tested on thiol compounds with varying molecular weights, including Cys derivatives, Cys-containing peptides, and reduced bovine serum

SUBMITTER: Yamaguchi H 

PROVIDER: S-EPMC11820211 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature