A Colorimetric and Fluorescent Dual-Mode Sensor Based on a Smartphone-Assisted Laccase-like Nanoenzyme for the Detection of Tetracycline Antibiotics.

ABSTRACT: A copper-based nanoenzyme (Cu-BL) co-modified by L-L-lysine and 2-2-amino terephthalic acid has laccase-like activity and fluorescence characteristics. Based on this, a colorimetric and fluorescent dual-mode sensor was developed to visually and quantitatively detect tetracycline antibiotics (TCs), including tetracycline (TC), chlortetracycline (CTC), and oxytetracycline (OTC). In the colorimetric detection system, TCs can inhibit the generation of singlet oxygen (¹O₂) and weaken the ability of 2,4-dichlorophenol (2,4-DP) to be oxidized into pink-colored quinone substances. The linear ranges are 0.5-80 μM, 1-80 μM, and 0.25-80 μM, and the detection limits are 0.27 μM, 0.22 μM, and 0.26μM, respectively. In addition, due to the inner filter effect, tetracycline antibioti

SUBMITTER: Chen H 

PROVIDER: S-EPMC11820295 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature