ABA and Pre-Harvest Sprouting Differences in Knockout Lines of OsPHS3 Encoding Carotenoid Isomerase via CRISPR/Cas9 in Rice.

ABSTRACT: We generated and characterized knockout mutant lines of the OsPHS3 gene using the CRISPR/Cas9 system. The knockout lines of the OsPHS3 gene showed that 1 bp and 7 bp deletion, early termination codons were used for protein production. Agronomic characteristics of knock-out lines were reduced in plant height, culm diameter, panicle length, seed size and weight, except for the number of tillers. In addition, we analyzed the expression levels of carotenoid biosynthesis genes by qRT-PCR. Among the genes encoding carotenoid metabolic pathway enzymes, the level of transcripts of PSY1, PSY2, PSY3, PDS and ZDS were higher in the KO lines than in the WT line. In contrast, transcription of the ε-LCY, β-LCY and ZEP1 genes we

SUBMITTER: Jung YJ 

PROVIDER: S-EPMC11820545 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature