Rapid Detection of Alpha-Fetoprotein (AFP) with Lateral Flow Aptasensor.

ABSTRACT: We present a lateral flow aptasensor for the visual detection of alpha-fetoprotein (AFP) in human serum. Leveraging the precise molecular recognition capabilities of aptamers and the distinct optical features of gold nanoparticles, a model system utilizing AFP as the target analyte, along with a pair of aptamer probes, is implemented to establish proof-of-concept on standard lateral flow test strips. It is the first report of an antibody-free lateral flow assay using aptamers as recognition probes for the detection of AFP. The analysis circumvents the numerous incubation and washing steps that are typically involved in most current aptamer-based protein assays. Qualitative analysis involves observing color changes in the test area, while quantitative data are obtained by measuring the opti

SUBMITTER: Ma M 

PROVIDER: S-EPMC11820797 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature