Optimization of Gelatin and Crosslinker Concentrations in a Gelatin/Alginate-Based Bioink with Potential Applications in a Simplified Skin Model.

ABSTRACT: Three-dimensional bioprinting allows for the fabrication of structures mimicking tissue architecture. This study aimed to develop a gelatin-based bioink for a bioprinted simplified skin model. The bioink printability and chemical-physical properties were evaluated by varying the concentrations of gelatin (10, 15, and 20%) in a semi-crosslinked alginate-based bioink and calcium chloride (100, 150, and 200 mM) in post-printing crosslinking. For increasing the gelatin concentration, the gelatin-based formulations have a shear thinning behavior with increasing viscosity, and the filament bending angle increases, the spreading ratio value approaches 1, and the shape fidelity and the printing resolution improve. However, the formulation containing 20% of gelatin was not homogeneous, resulting al

SUBMITTER: Cavallo A 

PROVIDER: S-EPMC11820930 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature