Allelic Analysis of the <i>Gli-B1</i> Locus in Hexaploid Wheat Using Reverse-Phase-Ultra-Performance Liquid Chromatography.

ABSTRACT: Wheat (Triticum aestivum L.) omega-5 gliadin, a major allergen responsible for wheat-dependent exercise-induced anaphylaxis in humans, is encoded by genes located at the Gli-B1 locus on chromosome 1B, which exhibits genetic polymorphism. Gli-B1 alleles have generally been identified based on the electrophoretic mobilities of the encoded gamma-, omega-1,2, and omega-5 gliadins in acid polyacrylamide gel electrophoresis. However, the similar mobilities of omega-5 gliadin variants make it difficult to distinguish them among different wheat varieties. In this study, we optimized reverse-phase-ultra-performance liquid chromatography (RP-UPLC) conditions to separate omega-5 gliadins in the reference wheat cultivar Chinese Spring and its nullisomic-tetrasomic lines for chromo

SUBMITTER: Lee JY 

PROVIDER: S-EPMC11821039 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature