High-Resolution LC-MS Simultaneous Quantification of Forty-Six Compounds from <i>Jatropha podagrica</i> Fruit Recommends Four Top Antioxidant Contributors as Q-Markers.

ABSTRACT: There has been no chemical analysis of the fruit of medicinal plant Jatropha podagrica until now. The current study aimed to qualitatively and quantitatively analyze the J. podagrica fruit using a high-resolution LC-MS strategy, i.e., library-comparison ultra-high-performance liquid chromatography-Quadrupole-Orbitrap-tandem mass spectrometry. The strategy putatively identified 46 compounds from fresh fruit. During the putative identification, 10 isomers (e.g., (vitexin vs. isovitexin) were completely distinguished from each other. Thereafter, all 46 compounds were simultaneously quantified using authentic standard comparison method. Finally, they were also subjected to the 2,2'-azino bis (3-ethylbenzothiazolin-6-sulfonic acid radical (ABTS^+•)-scavenging assay to ch

SUBMITTER: Cai R 

PROVIDER: S-EPMC11821128 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature