Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro.

ABSTRACT:

Introduction

Early preimplantation embryos of mammals exhibit pronounced sensitivity to hyperosmotic conditions, which results in an embryonic developmental block. The reduction of medium osmolarity or the supplementation with organic osmolytes can eliminate this arrest. Therefore, cell volume homeostasis is essential for embryonic development in vitro. Oocytes become capable of independent volume regulation after detaching from the follicle microenvironment. Whether the efficiency and quality of oocyte maturation can be improved by optimizing cell volume regulation by adjusting the osmolality of the culture medium in the presence of the organic osmolyte of glycine remains to be determined.

Methods

The IVM of porcine oocytes was divided into two stages, i.e. the first

SUBMITTER: Liu L 

PROVIDER: S-EPMC11821615 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature