Gene knockout studies of Dps protein reveals a novel role for DNA-binding protein in maintaining outer membrane permeability.

ABSTRACT: DNA-binding proteins like Dps are crucial for bacterial stress physiology. This study investigated the unexpected role of Dps protein in maintaining outer membrane integrity of Salmonella Typhimurium. We observed that a Δdps mutant displayed increased sensitivity to glycopeptide antibiotics (vancomycin, nisin), which are ineffective against Gram-negative bacteria due to their thick outer membrane (OM). Furthermore, the Δdps mutant exhibited susceptibility to membrane-disrupting agents like detergents (deoxycholate, SDS) and phages. The perforation was observed in OM after the treatment of vancomycin using atomic force microscopy. Notably, this sensitivity was rescued by supplementing the media with calcium and magnesium cations. These findings suggest a novel function for Dps in maintainin

SUBMITTER: Pant I 

PROVIDER: S-EPMC11821673 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature