The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.

ABSTRACT:

Background

Low-temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In flow cytometry data analysis, traditional manual "gating" is susceptible to past experience and faces the challenge of manual subjective bias, time-consuming, and multidimensional data analysis. With the development of algorithms, the advantages of dimensionality reduction and clustering in result analysis are gradually becoming more prominent.

Methods

Flow cytometry was used to detect the effects of cryopreservation and freeze-thaw cycle on T-cell subsets, and to analyze the data using automated algorithmic analysis and conventional manual "gating" methods.

Results

The results showed that the number and viability of

SUBMITTER: Min Q 

PROVIDER: S-EPMC11821723 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature