Anti-inflammatory effects of rutin in lipopolysaccharide-stimulated canine macrophage cells.

ABSTRACT:

Background/objectives

Inflammatory responses are key pathological factors in various canine diseases, making the control of inflammatory responses vital for canine health. This study examined the anti-inflammatory effects of rutin on DH82 cells, a type of canine macrophage, against lipopolysaccharide (LPS)-induced inflammatory responses.

Materials/methods

The inflammatory in vitro experimental model was established by stimulating canine macrophage DH82 cells with LPS. To evaluate the inflammation-preventative effects of rutin, analyses were conducted using enzyme-linked immunosorbent assay, western blot, and real-time quantitative reverse transcription polymerase chain reaction.

Results

Rutin inhibited the LPS-induced increase in the protein and gene levels of

SUBMITTER: Jang H 

PROVIDER: S-EPMC11821770 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature