Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis.

ABSTRACT: Bacillus subtilis uses cytoplasmic complexes called stressosomes to initiate the σ^B-mediated general stress response to environmental stress. Each stressosome comprises two types of proteins - RsbS and four paralogous RsbR proteins - that are thought to sequester the RsbT protein until stress causes RsbT release and subsequent σ^B activation. RsbR proteins have been assumed to sense stress, but evidence for their sensing function has been elusive, and the identity of the true sensor has remained unknown. Here, we conduct an alanine-scanning analysis of the putative sensing domain of one of the RsbR paralogs, RsbRA. We find that single substitutions impact but do not abolish the σ^B response, suggesting that RsbRA has a key role in σ^B response dynam

SUBMITTER: Khadka R 

PROVIDER: S-EPMC11821858 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature