Transcriptional analysis of murine biliary atresia identifies macrophage heterogeneity and subset-specific macrophage functions.

ABSTRACT:

Introduction

Macrophages play an important role in disease progression of pediatric cholestatic liver disease, particularly biliary atresia (BA); however, the restorative versus pathogenic role for precise macrophage subsets remains poorly defined. We aimed to distinguish the transcriptional profiles and roles of defined macrophage subset(s) in murine BA.

Methods

We used multiparameter flow cytometry and RNA-sequencing analysis to profile recruited CD11b^hiCD64+ hepatic macrophages by cell surface expression of MHCII and Ly6c in the Rhesus rotavirus (RRV)-induced murine model of BA versus saline controls. Modulation of macrophage numbers via intra-peritoneal injections of clodronate-loaded liposomes was performed to determine the association between macrophage numb

SUBMITTER: Gromer KD 

PROVIDER: S-EPMC11821939 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature