Hyperspectral Imaging for High Throughput Optical Spectroscopy of pL Droplets.

ABSTRACT: Droplet-based microfluidics is a powerful tool for high-throughput analysis of liquid samples with significant applications in biomedicine and biochemistry. Nevertheless, extracting content-rich information from single picolitre-sized droplets at high throughputs remains challenging due to the weak signals associated with these small volumes. Overcoming this limitation would be transformative for fields that rely on high-throughput screening, enabling broader multiparametric analysis. Here we present an integrated optofluidic platform that addresses this critical point by combining advanced hyperspectral imaging with self-referencing and measurement automation. With this approach our platform achieves high temporal and spectral resolution with shot-noise limited performance, allowing for t

SUBMITTER: Sulliger M 

PROVIDER: S-EPMC11822737 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature