Coupling of Size Exclusion Chromatography to High Throughput Charge Detection Mass Spectrometry for the Analysis of Large Proteins and Virus-like Particles.

ABSTRACT: Charge detection mass spectrometry (CD-MS) is an emerging single-particle technique where both the m/z and charge are measured individually to determine each ion's mass. It is particularly well-suited for analyzing high mass and heterogeneous samples. With conventional MS, the loss of charge state resolution with high mass samples has hindered the direct coupling of MS to separation techniques like size exclusion chromatography (SEC) and forced the use of lower resolution detectors. Here, we show how CD-MS, leveraging high-throughput methods such as multiple ion charge extraction (MICE), can match the time scale of SEC, extending the samples amenable to separation analysis by SEC-CD-MS into the megadalton regime and beyond. As part of this work, we have developed low flow ult

SUBMITTER: Parikh RA 

PROVIDER: S-EPMC11822739 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature