Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family.

ABSTRACT: Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and spindle pole bodies in yeast. The ciliate Tetrahymena thermophila contains at least four centrin genes as determined by sequence homology, and these have distinct localization and expression patterns. CEN1's role at the basal body was examined more closely. The Cen1 protein localizes primarily to two locations: one is the site at the base of the basal body where duplication is initiated. The other is the transition zone between the basal body and axoneme. CEN1 is an essential gene, the deletion of which results in the loss of basal bodies, which is likely due to defects in both basal body duplicati

SUBMITTER: Stemm-Wolf AJ 

PROVIDER: S-EPMC1182301 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature