The tumour microenvironment of pilocytic astrocytoma evolves over time via enrichment for microglia.

ABSTRACT: Pilocytic astrocytoma (PA) is the commonest low-grade tumour affecting children and is frequently experienced as a chronic disease associated with extended treatment, periods of regrowth, and long-term disability. This contrasts with the view of PA as a benign tumour with positive clinical outcomes and raises the fundamental question of biologically driven change over time within these tumours, which will impact diagnosis, stratification, and management. To investigate the molecular, cellular, and pathological stability of PA we performed RNA sequencing, methylation array profiling, immunohistochemistry, and targeted panel DNA sequencing on a cohort of 15 PA patients with matched primary/longitudinal samples at a mean sampling interval of 2.7 years. Through pairwise analysis of primary ver

SUBMITTER: Stone TJ 

PROVIDER: S-EPMC11823165 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature