Unraveling Human Hepatocellular Responses to PFAS and Aqueous Film-Forming Foams (AFFFs) for Molecular Hazard Prioritization and In Vivo Translation.

ABSTRACT: Aqueous film-forming foams (AFFFs) are complex product mixtures that often contain per- and polyfluorinated alkyl substances (PFAS) to enhance fire suppression and protect firefighters. However, PFAS have been associated with a range of adverse health effects (e.g., liver and thyroid disease and cancer), and innovative approach methods to better understand their toxicity potential and identify safer alternatives are needed. In this study, we investigated a set of 30 substances (e.g., AFFF, PFAS, and clinical drugs) using differentiated cultures of human hepatocytes (HepaRG, 2D), high-throughput transcriptomics, deep learning of cell morphology images, and liver enzyme leakage assays with benchmark dose analysis to (1) predict the potency ranges for human liver injury, (2) delineate gene- a

SUBMITTER: Mauge-Lewis KA 

PROVIDER: S-EPMC11823446 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature