<i>In vitro</i> potentiation of tetracyclines in <i>Pseudomonas aeruginosa</i> by RW01, a new cyclic peptide.

ABSTRACT: The pipeline for new drugs against multidrug-resistant Pseudomonas aeruginosa remains limited, highlighting the urgent need for innovative treatments. New strategies, such as membrane-targeting molecules acting as adjuvants, aim to enhance antibiotic effectiveness and combat resistance. RW01, a cyclic peptide with low antimicrobial activity, was selected as an adjuvant to enhance drug efficacy through membrane permeabilization. RW01's activity was evaluated via antimicrobial susceptibility testing in combination with existing antibiotics on 10 P. aeruginosa strains and analog synthesis. Synergy was assessed using checkerboard assays, and one-step mutants were generated to identify altered pathways through whole-genome sequencing and variant analysis. Permeabilizing activity w

SUBMITTER: Roson-Calero N 

PROVIDER: S-EPMC11823630 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature