Genetic variation in microRNA-binding site and prognosis of patients with colorectal cancer.

ABSTRACT:

Background

Single nucleotide polymorphisms (SNPs) located in the 3'-UTR of miRNA target genes could affect miRNA-mediated gene regulation, thereby contributing to the susceptibility or prognosis of cancer. Accordingly, the present study analyzed SNPs located at putative miRNA-binding sites of the 3'-UTR of various genes and investigated their impact on the prognosis for patients with colorectal cancer.

Materials and methods

In total, 831 consecutive patients (discovery cohort, n = 309; validation cohort, n = 522) with curatively resected colorectal adenocarcinoma were enrolled. Plus, 157 SNPs were selected from an in silico analysis based on several miRNA and HapMap databases. The SNP genotyping was performed using a Sequenom MassARRAY. A luciferase assay was used to investi

SUBMITTER: Kim JG 

PROVIDER: S-EPMC11823891 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature