LncRNA and mRNA interaction study based on transcriptome profiles reveals potential core genes in the pathogenesis of human glioblastoma multiforme.

ABSTRACT:

Objectives

To study the expression profiles of lncRNA and mRNA in glioblastoma multiforme (GBM) and to find potential core genes in the pathogenesis of this high malignant disease.

Methods

Agilent Microarray (Arrystar v2.0) was used to detect the expressions of 33,045 lncRNAs and 30,215 coding transcripts in 5 GBM and 5 normal brain samples. Differentially expressed lncRNAs and mRNAs were identified through Volcano Plot filtering. The expressions of six lncRNAs were further detected by qPCR to validate the results of microarray. The function of differential mRNA was determined by pathway and GO analysis, and the function of lncRNAs was studied by subgroup analysis and by their physical or functional relationships with corresponding mRNAs.

Results

A total of 815 lncRNA

SUBMITTER: Yan Y 

PROVIDER: S-EPMC11824101 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature