The Chinese herb polyphyllin D sensitizes ovarian cancer cells to cisplatin-induced growth arrest.

ABSTRACT:

Purpose

We evaluated the effects of polyphyllin D (PD), a natural compound with anti-neoplastic activity and a major component of the Chinese herb Paris polyphylla, on ovarian cancer (OVCA) cell line proliferation and platinum sensitivity.

Methods

A panel of 20 OVCA cell lines was subjected to PD treatment, MTS proliferation assays, and determination of IC50. Pre-treatment, baseline genome-wide Affymetrix expression analysis was performed on each cell line, and Pearson's correlation was performed to identify genes associated with OVCA PD sensitivity. Twelve cell lines were treated with PD with and without cisplatin, and the effects of PD on cisplatin IC50 were quantified. Genes associated with OVCA PD sensitivity were evaluated for associations with survival in a publically

SUBMITTER: Al Sawah E 

PROVIDER: S-EPMC11824142 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature