KexB Gene Correlates With Aspergillus flavus Keratitis Severity: A Whole-Genome Analysis.

ABSTRACT:

Purpose

Fungal keratitis caused by Aspergillus flavus (A. flavus) can result in severe inflammation and corneal stromal melting, leading to visual impairment. This study aimed to identify virulent genes correlated with the severity of A. flavus keratitis using whole-genome sequencing.

Methods

Whole-genome sequencing of 21 clinical A. flavus strains from cornea was performed to elucidate the pathogenesis of A. flavus in infectious keratitis, followed by pan-genome analysis and virulence analysis. To further understand the results from the previous analyses, growth phenotypes and virulence effect of mutant strains were validated experimentally, including the spore counting, growth pattern under different conditions, and clinical and pathological evaluation of A. flavus keratit

SUBMITTER: Liu J 

PROVIDER: S-EPMC11824512 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature