Visualization of Small Vessels by Micro-Computed Tomography Using Titanium Dioxide Nanoparticles as a Novel Contrast Agent.

ABSTRACT: Angiography by means of micro-computed tomography (m-CT) is extensively used for the diagnosis of vasculature disorders. To establish a connection between m-CT images and genuine histopathology findings, we developed two novel titanium dioxide nanoparticle (TiO₂-NP)-based perfusion contrast agents: TiNpCA-1 and TiNpCA-2. Three-dimensionally reconstructed m-CT images in mice perfused with these contrast agents showed high resolution and accuracy in various organs without deformation or dilation of vessels. Vessels < 20  μm in diameter were clearly visualized by m-CT, and capillaries of 4 μm in diameter were visualized by nano-CT. After perfusion, the contrast agents were kept in the vessels by the formation of an aggregate with ethanol. Histological samples were pre

SUBMITTER: Goto T 

PROVIDER: S-EPMC11824794 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature