Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy.

ABSTRACT:

Graphical abstract

The chromosomal status of Day 5 blastocysts was determined based on trophectoderm biopsy for all 24 chromosomes by array comparative genomic hybridization (array-CGH). The blastocoel fluid (BF) was extruded and amplified, and DNA concentration was read by a Qubit fluorometer. Apoptotic gene expression (TNFRSF10B, CASP2, BAX and CASP3) was analyzed by real-time quantitative PCR (RT-qPCR). Our findings suggest BF-DNA may be released in the blastocoel cavity of aneuploidy blastocysts under the influence of the apoptosis mechanism, and it appears that quantifying BF-DNA has the potential to aid in the selection of viable embryos.

Abstract

It is believed that aneuploid embryos release cell-free DNA (cfDNA) into the blastocyst cavity during the self-correction p

SUBMITTER: Khajehoseini F 

PROVIDER: S-EPMC11825185 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature