Folate depletion impact on the cell cycle results in restricted primary root growth in Arabidopsis.

ABSTRACT: Folates are vital one carbon donors and acceptors for a whole range of key biochemical reactions, including the biosynthesis of DNA building blocks. Plants use one carbon metabolism as a jack of all trades in their growth and development. Depletion of folates impedes root growth in Arabidopsis thaliana, but the mechanistic basis behind this function is still obscure. A global transcriptomic study hinted that folate depletion may cause misregulation of cell cycle progression. However, investigations on a direct connection thereof are scarce. We confirmed the effect of methotrexate (MTX), a folate biosynthesis inhibitor, on the expression of cell cycle genes. Subsequently, we determined the effect of MTX on root morphology and cell cycle progression through phase-specific cell cycle reporter

SUBMITTER: De Lepeleire J 

PROVIDER: S-EPMC11825618 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature