Introducing AVAC as an ultra-sensitive platform with broad dynamical range for high-throughput multiplexed biomarker detection using digital counting of plasmonic nanoparticles.

ABSTRACT: Accurate detection and quantification of biomarkers at ultra-low levels is critical for disease diagnosis and effective treatment. Traditional detection technologies often lack the sensitivity, specificity, throughput, or multiplexing capacity required for comprehensive diagnostics, providing only a subset of these requirements. Here, we introduce AVAC, an automated optical technology for rapid and accurate biomarker detection with ultra-high sensitivity that significantly outperforms standard clinical assays. The core of this technology is the digital counting of plasmonic nanoparticles used as optical labels, enabling multiplexed, high-throughput detection of biomarkers. Validation studies demonstrate AVAC's high accuracy, with 98.2% specificity and detection limits as low as 26 fg/mL fo

SUBMITTER: Cebrian V 

PROVIDER: S-EPMC11825739 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature