MiRNA sequencing identifies immune-associated miRNAs and highlights the role of miR-193b-5p in sepsis and septic shock progression.

ABSTRACT: This study aimed to evaluate the diagnostic value of miR-193b-5p and miR-511-5p in sepsis and septic shock from the perspective of immune regulation. Initially, serum exosomal miRNA sequencing was conducted on patients with sepsis (n = 6), septic shock (n = 6), and healthy controls (n = 3). Using bioinformatics analysis, miR-193b-5p and miR-511-5p were identified as immune-related miRNAs differentiating sepsis from septic shock. Subsequently, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the identified miRNAs in a cohort of 90 participants, including 30 patients with sepsis, 30 with septic shock, and 30 general surgery patients serving as controls. The results indicated that miR-193b-5p expression level was significantly reduced in septic patients and

SUBMITTER: Li C 

PROVIDER: S-EPMC11825773 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature