Characterization of RNF144B and PPP2R2A identified by a novel approach using TCGA data in ovarian cancer.

ABSTRACT: TCGA has identified predominant somatic copy number alterations (SCNA) affecting numerous genes in HGSOC. To identify cancer-driver genes from the regions of SCNA, we have devised a scoring system that integrates information from different genetic alterations. Applying this scoring system to the TCGA-HGSOC dataset (n = 316) we have identified several well-known and novel putative cancer genes in HGSOC. We functionally validated the roles of two previously unknown genes, RNF144B and PPP2R2A. RNF144B, an E3 ubiquitin-ligase is amplified and overexpressed in 16% of HGSOC (TCGA). Overexpression of RNF144B in ovarian cancer cells increased cell proliferation, colony formation, and migration. RNF144B was significantly overexpressed in 50% of primary tumors from patients with HGSOC compared to th

SUBMITTER: Manasa P 

PROVIDER: S-EPMC11825944 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature