A combinatorial culture strategy to develop pseudomyxoma peritonei organoid models.

ABSTRACT:

Background and objectives

Few preclinical models of pseudomyxoma peritonei (PMP) have been developed, probably due to the tumor's low incidence and its peculiar characteristics of slow growth. Therefore, there is a need to develop more refined PMP models that better reflect its characteristics. The aim of the study is to develop a culture strategy to generate organoid models derived from PMP patient samples.

Methods

We followed a strategy based on combinatorial culture conditions that include the different factors essential for PMP growth and that mimic the microenvironment present in the patients.

Results

We cultured PMP samples in the presence of the various factors produced by the niche environment of PMP. We obtained 12 PMP organoid models, each of which grows und

SUBMITTER: Varinelli L 

PROVIDER: S-EPMC11826015 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature