Biosensor characterization: formal methods from the perspective of proteome fractions.

ABSTRACT: Many studies characterize transcription factors and other regulatory elements to control gene expression in recombinant systems. However, most lack a formal approach to analyse the inherent and context-specific variations of these regulatory components. This study addresses this gap by establishing a formal framework from which convenient methods are inferred to characterize regulatory circuits. We modelled the bacterial cell as a collection of proteome fractions. Deriving the time-dependent proteome fraction, we obtained a general theorem that describes its change as a function of its expression fraction, a specific portion of the total biosynthesis flux of the cell. Formal deduction reveals that when the proteome fraction reaches a maximum, it becomes equivalent to its expression fractio

SUBMITTER: Vaccari NA 

PROVIDER: S-EPMC11826058 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature