Inhibition of diacylglycerol lipase α induced blood-brain barrier breach in female Sprague-Dawley rats.

ABSTRACT: The endocannabinoid system's significance in maintaining blood-brain barrier (BBB) integrity under physiological and pathological conditions is suggested by several reports, but the underlying molecular mechanisms are not well understood. In this paper, we investigated the effects of depletion of 2-arachidonoylglycerol (2-AG), one of the main endocannabinoids in the central nervous system, on BBB integrity using pharmacological tools. Female Sprague-Dawley rats were injected with the diacylglycerol lipase α (DAGLα) inhibitor LEI-106 (40 mg/kg, i.p.), followed by assessment of BBB integrity via in situ brain perfusion. Liquid chromatography-mass spectrometry, western immunoblotting, light transmittance experiments and pressure myography were also used to further examine the results of DAGLα

SUBMITTER: Liktor-Busa E 

PROVIDER: S-EPMC11826068 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature