Safety evaluation of the food enzyme cyclomaltodextrin glucanotransferase from the non-genetically modified <i>Anoxybacillus caldiproteolyticus</i> strain AE-KCGT.

ABSTRACT: The food enzyme cyclomaltodextrin glucanotransferase ((1-4)-α-d-glucan 4-α-d-[(1-4)- α-d-glucano]-transferase; EC 2.4.1.19) is produced with the non-genetically modified Anoxybacillus caldiproteolyticus strain AE-KCGT by Amano Enzyme Inc. The food enzyme is free from viable cells of the production organism. It is intended to be used in two food manufacturing processes. The dietary exposure was estimated to be up to 20.27 mg total organic solids (TOS)/kg bw per day in European populations. Genotoxicity tests did not raise safety concerns. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 2246 mg TOS/kg bw per day, the highest dose tested, which when compared with the estimated

SUBMITTER: EFSA Panel on Food Enzymes (FEZ) 

PROVIDER: S-EPMC11826218 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature