IDEP-based single-cell isolation in a two-dimensional array of chambers addressed by easy-to-align wireless electrodes.

ABSTRACT: Platforms capable of selective single-cell capture and enclosure in a fluidically isolated volume for subsequent analysis are crucial for unmasking cellular heterogeneity. Our research group has previously reported an approach that employs wireless bipolar electrodes (BPEs) to facilitate individual isolation of cells in large arrays of pico- to nanoliter scale chambers by dielectrophoresis (DEP). This device was leveraged for a single-cell enzymatic assay and the isolation of circulating tumor cells (CTCs) from patient-derived blood samples, which takes advantage of the selectivity of DEP. However, alignment of BPEs to the microchamber openings is nontrivial, and augmentation of the array dimensions accumulates alignment error, thereby disrupting the uniformity of cell capture across the d

SUBMITTER: Rathnaweera TN 

PROVIDER: S-EPMC11826382 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature