The Ebola virus genomic replication promoter is bipartite and follows the rule of six.

ABSTRACT: In this work we investigated the cis-acting signals involved in replication of Ebola virus (EBOV) genomic RNA. A set of mingenomes with mutant 3' ends were generated and used in a reconstituted replication and transcription system. Our results suggest that the EBOV genomic replication promoter is bipartite, consisting of a first element located within the leader region of the genome and a second, downstream element separated by a spacer region. While proper spacing of the two promoter elements is a prerequisite for replication, the nucleotide sequence of the spacer is not important. Replication activity was only observed when six or a multiple of six nucleotides were deleted or inserted, while all other changes in length abolished replication completely. These data indicate that the EBOV r

SUBMITTER: Weik M 

PROVIDER: S-EPMC1182658 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature