CAYSS: Package for Automatic Cytometry Analysis of Yeast Spore Segregation.

ABSTRACT: Meiotic recombination is a powerful source of haplotypic diversity, and thus plays an important role in the dynamics of short-term adaptation. However, high-throughput quantitative measurement of recombination parameters is challenging because of the large size of offspring to be genotyped. One of the most efficient approaches for large-scale recombination measurement is to study the segregation of fluorescent markers in gametes. Applying this to yeast spores by flow cytometry has already been proved to be highly efficient, but manual analyses of distributions of signal intensities is time-consuming and produces nonperfectly reproducible results. Such analyses are required to identify events corresponding to spores and to assign each of them to a genotypic class depending on their fluoresc

SUBMITTER: Raffoux X 

PROVIDER: S-EPMC11826985 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature