Utility of methylated DNA markers for the diagnosis of malignant biliary strictures.

ABSTRACT:

Background and aims

Early identification of malignant biliary strictures (MBSs) is challenging, with up to 20% classified as indeterminants after preliminary testing and tissue sampling with endoscopic retrograde cholangiopancreatography. We aimed to evaluate the use of methylated DNA markers (MDMs) from biliary brushings to enhance MBS detection in a prospective cohort.

Approach

Candidate MDMs were evaluated for their utility in MBS diagnosis through a series of discovery and validation phases. DNA was extracted from biliary brushing samples, quantified, bisulfite-converted, and then subjected to methylation-specific droplet digital polymerase chain reaction.  Patients were considered to have no malignancy if the sampling was negative and there was no evidence of malignancy

SUBMITTER: Cooley MA 

PROVIDER: S-EPMC11827039 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature