Involvement of icaritin in the regulation of osteocyte exosomal microRNAs.

ABSTRACT:

Objective

To explore the effects of Icaritin (ICA) on the regulation of osteocyte exosomal miRNAs and to promote the understanding of the potential molecular mechanisms involved in bone repair by ICA.

Methods

MLO-Y4 cells were treat with PBS or 10 µM ICA for 24 h and the supernatant was collected. Exosomes were isolated and purified according to standard methods, and identified by transmission electron microscopy, nanoparticle tracking analysis and protein blotting. Exosomal miRNAs were analysed by RNA sequencing.

Results

Osteocyte exosomes were successfully isolated and characterised. MiRNA sequencing showed that two known exosomal miRNAs (miR-128-3p, miR-30a-5p) were significantly up-regulated and two were significantly down-regulated (miR-5112, miR-1285) after ICA

SUBMITTER: Zhang K 

PROVIDER: S-EPMC11827220 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature