A novel approach to digital characterisation of Tertiary Lymphoid Structures in colorectal cancer.

ABSTRACT:

Introduction

Tertiary Lymphoid Structures (TLS) in cancer tissue are potential sites for the organisation of immune responses to cancer, and correlate positively with improved clinical outcomes for patients including in colorectal cancer (CRC). However it has proven challenging to standardise assessment of TLS due to the highly variable appearances of circumscribed domains of TLS within tissue sections. A recent three-dimensional reconstruction of TLS in CRC tissue showed that TLS are often large, multi-lobular structures, suggesting that assessing TLS across whole sections may be necessary to provide an accurate view of TLS activity in a patient's tumour.

Methods

We used whole-section scans of multiplexed immunofluorescence images to characterise TLS from 22 subjects with C

SUBMITTER: Munoz-Erazo L 

PROVIDER: S-EPMC11827424 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature