Integrated bulk and single-cell RNA sequencing to identify potential biomarkers in intervertebral disc degeneration.

ABSTRACT:

Background

Nucleus pulposus (NP) deterioration plays a significant role in the development of intervertebral disc degeneration (IVDD) and low back pain (LBP). This paper aims to identify potential genes within degenerated NP tissue and elucidate the pathogenesis of IVDD through bioinformatics analysis.

Methods

We conducted a transcriptomic analysis of patient's degenerative NP tissue employing advanced bioinformatics techniques and machine learning algorithms. Utilizing hdWGCNA, we successfully acquired WGCNA single-cell sequencing data and pinpointed crucial genes implicated in IVDD. Subsequently, we employed the Monocle3 package to perform pseudotime sequence analysis, enabling the identification of genes associated with the differentiation and developmental processes of N

SUBMITTER: Fan C 

PROVIDER: S-EPMC11827443 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature