Diverse effects of coronavirus-defective viral genomes on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication.

ABSTRACT:

Background

The mechanism by which coronavirus-defective viral genomes (DVGs) affect coronavirus and host cells during infection remains unclear. A variety of DVGs with different RNA structures can be synthesized from coronavirus-infected cells, and these DVGs can also encode proteins. Consequently, in the present study, we first dissected the effects of individual DVGs on the synthesis of IFNβ and ISG15 mRNAs at the RNA, protein and combined levels, and then examined whether different coronavirus-DVGs have different effects on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication both individually and collectively under different infection conditions.

Methods

To dissect the effects of individual DVGs on the synthesis of IFNβ and ISG15 mRNAs at the RNA, protein an

SUBMITTER: Hsu HW 

PROVIDER: S-EPMC11827481 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature