Genomic aberrations in hepatocellular carcinoma related to osteopontin expression detected by array-CGH.

ABSTRACT:

Purpose

We have demonstrated that overexpression of osteopontin (OPN) could contribute to metastasis in hepatocellular carcinoma (HCC), and that OPN-positive cancer cells are often localized in the periphery of cancer nodules adjacent to stromal cells. This study was to identify the difference of intratumor genomic aberrations between OPN-positive and OPN-negative HCC cells.

Methods

Immunohistochemical staining for OPN was performed in both archival and fresh HCC tumor tissues. Seven cases of OPN-positive HCC were chosen for laser capture microdissection. The OPN-positive and OPN-negative cancer cells were captured separately from serial frozen sections. Genomic DNA was extracted and quantified. Microarray-based comparative genomic hybridization (array-CGH) was used to achie

SUBMITTER: Wu JC 

PROVIDER: S-EPMC11828040 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature