Quantitative investigation of photobleaching-based autofluorescence suppression in formalin-fixed paraffin-embedded human tissue.

ABSTRACT: Immunofluorescence (IF) is a crucial technique in histopathology, enabling the visualization of multiple antibody distributions within single tissue specimens. However, autofluorescence (AF), which originates from endogenous molecules in formalin-fixed paraffin-embedded (FFPE) tissue, poses a persistent challenge by interfering with the fluorescence signal of interest in IF analysis. While photo-irradiative bleaching has emerged as a protocol to suppress the AF signal, there have been minimal quantitative investigations into this method across various experimental conditions. In this study, we investigated the efficacy of a bleaching-based method for reducing AF in FFPE human tissues. This research analyzed AF intensity as a function of exposure time, deparaffinization, emission range, and

SUBMITTER: Hwang W 

PROVIDER: S-EPMC11828445 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature