Efficient, broadly tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy.

ABSTRACT: Three-photon fluorescence microscopy (3PM) has driven rapid progress in deep-tissue imaging beyond the depth limit of two-photon microscopy, with impacts in neuroscience, immunology, and cancer biology. Three-photon excitation places a premium on ultrashort pulses with high peak power in the 1300- and 1700-nm wavelength bands, which allow deepest imaging. The inefficiency and cost of current sources of these pulses present major barriers to the use of 3PM in biomedical research labs. Fiber sources of such pulses could potentially alleviate these problems, but the peak-power limitations of optical fibers have limited their use in 3PM. Here, we describe a fiber-based source of femtosecond pulses with multi-megawatt peak power. Femtosecond pulses at 1030 nm are launched into an antiresonant h

SUBMITTER: Eisenberg Y 

PROVIDER: S-EPMC11828453 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature