Establishment of one-step duplex TaqMan real-time PCR for detection of feline coronavirus and panleukopenia virus.

ABSTRACT: A comparative genomic analysis of feline coronavirus (FCoV) and feline panleukopenia virus (FPLV) was performed. Based on the conserved regions of the two viruses, specific probes and real-time PCR (qPCR) primers were designed, and a duplex TaqMan qPCR-based assay was established for detecting FCoV and FPLV. The results showed high analytical specificity, and no cross-response with other feline viruses was observed. This method is highly versatile and can be used to detect all FCoV strains stored in laboratories and recombinant plasmids constructed according to the sequences of blank FCoV strains in laboratories. The analytical sensitivity of this method in detecting FCoV and FPLV was as low as 50 copies/μL, which is approximately 20-fold greater than that of conventional PCR. The coeffici

SUBMITTER: Liu Z 

PROVIDER: S-EPMC11828816 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature